?url_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.relation=http%3A%2F%2Fwww.badankebijakan.kemkes.go.id%2Frepositori%2Fid%2Feprint%2F1907%2F&rft.title=Investigation+of+Potential+Animal+Reservoirs+for+Hepatitis+E+virus+in+East+Java%2C+Indonesia&rft.creator=Winoto%2C+Winoto&rft.subject=WC+500-590+Virus+Diseases&rft.description=Epidemic+HEV+transmission+was+recognized+in+Bondowoso%2C+East+Java+in+March+1998.+In+October+of+1999%2C+a+team+of+investigators+(from+NAMRU-2+and+BALITBANGKES)+returned+to+the+site+of+the+epidemic+to+conduct+field+sampling+of+blood%2C+stool+and+tissues+of+several+species+of+domestic+and+sylvatic+animals.++++++++The+purpose+of+this+investigation+was+two+fold%3A+1).+to+assess+reservoir+potential+for+various+animal+populations+in+the+maintenance+of+the+virus%2C+and+2).+Assess+risk+for+transmission+between+human+and+animals.+Sampling+was+conducted+at+the+same+villages+and+households+involved+in+the+1998+epidemic+as+well+as+at+a+control+village+that+was+not+involved+in+the+outbreak.+A+total+of+243+rodents+were+trapped%2C+euthanized+and+dissected+for+the+collection+of+tissue%2C+serum+and+feces.+Animals+sampled+for+sera+and+feces+included+411+chickens+(Gallus)%2C+and+191+domestic+bovines+(cow%2C+sheep+and+goat).+A+total+of+845+animals+were+sampled+for+this+study.+Analysis+of+sera+by+EIA+has+been+completed+with+result+5.84%25+of+Chicken%2C+2.78%25+of+cows%2C+7.14%25+of+goats%2C+1.82%25+of+sheep+and++2.78%25+of+rats+were+positive+to+HEV+IgG.+Shrews+were+tested+using+anti-mouse+conjugate%2C+and+3.17%25+were+positive.+Western+blot+was+performed+on+the+samples+to+confirm+the+AIA+result.+Sera+and+stool+were+also+analyzed+for+the+presence+of+HEV+nucleit+acids+(RNA)+using+reverse-transcriptase+polymerase+chain+reaction+(RT-PCR)%2C+but+all+of+them+were+negative.+RT-PCR+results+indicated+there+was+no+current+infection+of+HEV+among+those+animals+during+the+study+period%2C+but+the+positive+result+of+IgG+to+HEV+on+the+EIA+showed+Chicken%2C+cow%2C+goat%2C+sheep+rat+and+shrew+play+roll+in+the+maintenance+and+transmission+of+HEV+during+inter-epidemic+periods.&rft.publisher=Badan+Penelitian+dan+Pengembangan+Kesehatan&rft.date=2006&rft.type=Monograph&rft.type=NonPeerReviewed&rft.identifier=++Winoto%2C+Winoto++(2006)+Investigation+of+Potential+Animal+Reservoirs+for+Hepatitis+E+virus+in+East+Java%2C+Indonesia.++Project+Report.+Badan+Penelitian+dan+Pengembangan+Kesehatan.+++++