?url_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.relation=http%3A%2F%2Fwww.badankebijakan.kemkes.go.id%2Frepositori%2Fid%2Feprint%2F2704%2F&rft.title=PENELITIAN+LARVA+TOXORHYNCHITES+SEBAGAI+MEDIA+ISOLASI+VIRUS+DENGUE+DI+JAKARTA&rft.creator=Heriyanto%2C+Bambang&rft.subject=WC+500-590+Virus+Diseases&rft.description=Dengue+virus+infection+is+very+prevalent+in+Indonesia+and+is+getting+spread+to+other+area+and+potentially+to+other+countries.+++++++Dengue+infection+is+therefore+one+among+many+major+health+problem+which+should+be+properly+controlled.+One+problem+which+has+to+be+resolved+for+maximizing+the+above+mentioned+disease+control+and+historically+has+presented+certain+difficulties+to+the+arbovirus+laboratory+is+the+afficacy+of+identification+of+isolation+dengue+viruses.+++++++Suckling+mice+have+been+used+for+many+years+in+the+isolaation+of+dengue+viruses%2C+but+the+results+could+take-up+to+several+weeks+before+typing+results+were+known.+++++++The+use+vertebrate+cell+cultures+such+as+vero%2C+LLCCMK2+and+PS+cells+has+helped+to+spead+up+the+laboratory+diagnosis+of+dengue+infections%2C+but+the+method+is+technically+difficult%2C+expensive+and+requires+3+to+4+weeks+for+virus+identification+by+plaque+reduction+neutrlization+test.+++++++In+relation+to+thee+above+mentioned%2C+larvae+of+Toxorhynchites+mosquitoes+showed+promise+in+detection+of+dengue+type-2+antigen+following+intra+cerebral+inoculation+of+the+prototype+virus.+++++++The+introduction+of+mosquitoes+inoculation+for+dengue+diagnosis+has+improved+not+only+the+sensitivity+but+also+reduced+the+time+needed+for+isolation.&rft.publisher=Puslit+Penyakit+Menular&rft.date=1990&rft.type=Monograph&rft.type=NonPeerReviewed&rft.identifier=++Heriyanto%2C+Bambang++(1990)+PENELITIAN+LARVA+TOXORHYNCHITES+SEBAGAI+MEDIA+ISOLASI+VIRUS+DENGUE+DI+JAKARTA.++Project+Report.+Puslit+Penyakit+Menular.+++++