relation: http://www.badankebijakan.kemkes.go.id/repositori/id/eprint/5743/
title: IDENTIFIKASI TIPE HLA KELAS II DENGAN TEKNIK PCR
creator: Salwati, Ervi
subject: WC 1-100 Reference Works. General Works
description: HLA (Human Leukocyte Antigen) contains a set of genes located together on the short arm   of chromosome 6. These genes control immune responses, graft acceptance or rejection and  tumor surveillance. These abilities have close relationship with genetic variation (occur in  "many forms" or alleles) that bind and present antigens to T lymphocytes.  Using advanced technology and molecular biology approaches (PCR technique) detection  of genetic variation in the HLA region (or HLA typing) has been performed based on DNA .. PCR  is an in vitro technique to amplify the DNA sequence enzymatically. "Sequence Specific Primers"  (SSP) are designed for this PCR to obtain amplification of specific alleles or groups of alleles.  The PCR products are visualized through agarose gel electrophoresis stained with ethidium  bromide.  The PCR technique requires small amount of whole blood (0.5 - I ml), gives rapid,  accurate and complete result. This paper discuss identification of HLA class   typing using  PCR-SSP technique and show the examples of the results.
publisher: Sekretariat Badan Penelitian dan Pengembangan Kesehatan
date: 2002
type: Article
type: NonPeerReviewed
format: text
language: en
identifier: http://www.badankebijakan.kemkes.go.id/repositori/id/eprint/5743/1/154948-identifikasi-tipe-hla-kelas-ii-dengan-te-cfff662a.pdf
identifier:   Salwati, Ervi  (2002) IDENTIFIKASI TIPE HLA KELAS II DENGAN TEKNIK PCR.  Media Penelitian dan Pengembangan Kesehatan, 12 (1).  pp. 34-41.  ISSN 0853-9987