<mets:mets OBJID="eprint_5788" LABEL="Eprints Item" xsi:schemaLocation="http://www.loc.gov/METS/ http://www.loc.gov/standards/mets/mets.xsd http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-3.xsd" xmlns:mets="http://www.loc.gov/METS/" xmlns:mods="http://www.loc.gov/mods/v3" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance"><mets:metsHdr CREATEDATE="2026-07-05T13:05:38Z"><mets:agent ROLE="CUSTODIAN" TYPE="ORGANIZATION"><mets:name>Repositori BKPK</mets:name></mets:agent></mets:metsHdr><mets:dmdSec ID="DMD_eprint_5788_mods"><mets:mdWrap MDTYPE="MODS"><mets:xmlData><mods:titleInfo><mods:title>PERKEMBANGAN KOLONI PRIMER EMBRYONIC STEM CELL (ESC) MENCIT&#13;
PASCA VITRIFIKASI INNER CELL MASS (ICM)</mods:title></mods:titleInfo><mods:name type="personal"><mods:namePart type="given">Ratih</mods:namePart><mods:namePart type="family">Rinendyaputri</mods:namePart><mods:role><mods:roleTerm type="text">author</mods:roleTerm></mods:role></mods:name><mods:name type="personal"><mods:namePart type="given">Arief</mods:namePart><mods:namePart type="family">Boediono</mods:namePart><mods:role><mods:roleTerm type="text">author</mods:roleTerm></mods:role></mods:name><mods:abstract>Aplikasi maupun penelitian dasar untuk menggali potensi sel punca khususnya&#13;
pemanfaatan sel punca di bidang kesehatan terus dilakukan. Teknologi kriopreservasi&#13;
dibutuhkan untuk menjaga ketersediaan sel punca. Penelitian ini bertujuan mengamati&#13;
perkembangan koloni primer embryonic stem cell (ESC) mencit pasca vitrifikasi inner&#13;
cell mass (ICM). Inner Cell Mass (ICM) diperoleh dari embrio blastosis mencit betina&#13;
6ZLV�ZHEVWHU�PHQJJXQDNDQ� SUHJQDQW�PDUH¶V� VHUXP� JRQDGRWURSLQ� �306*�� GDQ� KXPDQ�&#13;
chorionic gonadotropin (hCG). ICM diisolasi dari embrio blastosis menggunakan metode&#13;
immunosurgery. ICM yang diperoleh dibagi menjadi dua kelompok yaitu ICM kontrol&#13;
dan ICM vitrifikasi. Kedua kelompok dikultur serta dilakukan pengamatan terhadap&#13;
tingkat perlekatan (attachment rate/AR) ICM, tingkat pembentukan koloni primer ESC&#13;
(primary colony/PC) dan diameter koloni primer ESC yang dikategorikan menjadi 3&#13;
kelompok yaitu kecil, sedang dan besar. Hasil menunjukkan bahwa ICM vitrifikasi&#13;
mempunyai persentase AR dan diameter koloni primer ESC pada kelompok sedang lebih rendah (p&lt;0,05) terhadap ICM kontrol. Namun persentase PC serta diameter koloni&#13;
primer ESC kelompok kecil dan besar pada ICM vitrifikasi tidak menunjukkan perbedaan&#13;
(P&gt;0,05) terhadap kontrol. Penelitian ini menunjukkan bahwa vitrifikasi dapat digunakan&#13;
untuk kriopreservasi ICM sebagai sumber ESC.</mods:abstract><mods:classification authority="lcc">QU Biochemistry. Cell Biology and Genetics</mods:classification><mods:originInfo><mods:dateIssued encoding="iso8601">2013-09</mods:dateIssued></mods:originInfo><mods:originInfo><mods:publisher>Badan Penelitian dan Pengembangan Kesehatan</mods:publisher></mods:originInfo><mods:genre>Article</mods:genre></mets:xmlData></mets:mdWrap></mets:dmdSec><mets:amdSec ID="TMD_eprint_5788"><mets:rightsMD ID="rights_eprint_5788_mods"><mets:mdWrap MDTYPE="MODS"><mets:xmlData><mods:useAndReproduction>
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