The isolation and characterization of chromosomal genes and bacterial plasmids are important for strain improvement to produce most valuable compounds on industrial scale. The isolation of the neutral protease (npr) fragment from Bacillus megaterium was carried out by polymerase chain reaction (PCR). The PCR experiments were done setting up of variation for oligonucleotide primers, annealing te…
A study to evaluate the potency of bacteriae from soil sample as biological Vector Control Research Station at Salatiga. Protein crystals were detected by Chilcott Wigley's method (1988). Samples of soil from 5 different locations in Kotamadya Salatiga, were evaluated. Total of 6 samples collected from 2 habitats (hole and branch of Euphoria longan), 1 samples from Butuh, 2 samples from Conde…
Bacillus thuringiensis H-14 was tested as follows : Laboratory trials revealed a LC50 at 3.2027 ppm and 1.4998 ppm and LC90 at 12.2521 ppm and 7.3356 ppm at exposures of respectively 24 and 48 hours. Small scale trials in ricefield plots in Hilisimaetano village showed a reduction rate of respectively 85.54 percent-89.54 percent, 88.36 percent-94.61 percent and 88.61 percent-94.55 percent at do…