Polymerase Chain Reaction (PCR) is a common method of creating copies of creating copies of specific fragments of DNA PCR rapidly amplifies a single DNA molecule into many billions of molecules and can be detected with visualization. Diagnostic of pleural effusion caused by Mycobacterium tuberculosis is still a clinical prili were oblem especially of low sensitivity in conventional methods. To …
The clinical diagnosis of thalassemia syndrome is not difficult to establish with relatively simple Investigations, but molecular study is needed to determine the genotype and for the prenatal diagnosis. In the molecular study, the DNA should be first amplifed and among the amplification techniques polymerase chain reaction is the simplest and the most rapid one. The procedure can in vitro ampl…
A major difficulty in some infectious diseases today is the time required to make a diagnosis. The gold standard has been culture and sensitivity (by means of microbiology investigation); however, some organisms are difficult to culture, and some are dangerous to grow or take long periods to grow and indentification. In these particular instances, a quicker and more accurate way to make a diagn…
The isolation and characterization of chromosomal genes and bacterial plasmids are important for strain improvement to produce most valuable compounds on industrial scale. The isolation of the neutral protease (npr) fragment from Bacillus megaterium was carried out by polymerase chain reaction (PCR). The PCR experiments were done setting up of variation for oligonucleotide primers, annealing te…