The brain is the body's vital organs are susceptible to damage. Have a high oxygen demand, is highly dependent on glucose, has a high metabolic rate, but have low adaptability of the injury and it is very difficult to regeneration. Injury on brain cells (neurons) is a serious condition because of risk for dysfunction and cells death. The brain needsfor oxygen and glycogen constanly to produce…
Isolation method to obtain pure BMVECs is hard to be done consistently and remains a challenge. In this study, we isolated BMVECs from Wistar rat and C571B16 mouse from Japan SLC. All procedures performed according to guidelines for the care and use of laboratory animals of Gunma University, Japan. The modification of isolation method was using bovine serum albumin (BSA) gradation, not Dextran-…