Zingiber officinate, Rosc, is a well known plat which can be used for the treatment of various diseases, and has been reported as an effective medicinal plant against Dirofilaria immitis in animal. The volatile oil and the pungent of Zingiber officinate, Rosc. have been effectively used larvae stage 3 (L3) B. malayi in vitro study. This study was designed to investigate the effect of the volati…